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10. the tubes of tritiated precursors should be prepared ahead of time and kept on ice. 5–10 min before use, the tube is placed into the 30 water bath shaker, to prewarm it to 30 . flask or tube should be removed from the shaker for the minimum time necessary to make the transfer, as it is important to main tain temperature and aeration.
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Table 1 transformability of released rp4 from naclotreated arb (10 5 − 10 6 cfuml) to chemically competent escherichia coli dh5α at ph 7.2, 20 c. full size table.
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Bacterial strains and media. the bacterial strains and plasmids used in this study are described in table strains were routinely grown in luriabertani (lb) broth at 37 c in an orbital shakerincubator and were maintained at −80 c in lb broth containing 15 (volvol) glycerol.
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Xanthomonas contains a large group of plant‐associated species, many of which cause severe diseases on important crops worldwide. six gluconate‐operon repressor (gntr) family transcriptional regulators are predicted in xanthomonas, one of which, belonging to the ytra subfamily, plays a prominent role in bacterial virulence. however, the direct targets and.
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Introduction. azorhizobium caulinodans ors571 is a microsymbiont of a tropical legume, sesbania rostrata, and is able to fix nitrogen in both the freeliving and symbiotic states (15–17).nitrogenfixing nodules are formed by a. caulinodans on the stems, as well as on the roots, of s. nodules occur at the sites of adventitious root primordia located on.
It was found that fragment rp4 hind iii stabilized puc18 as effectively as the full rp4 region. a series of subclones of rp4 hind iii was amplified by pcr, using primers listed in table 1 . each pcr product was cloned in puc18 with the aid of eco r i and bam h i restriction sites and amplified a subsection of rp4 hind iii .
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Introduction. it is generally accepted that horizontal gene transfer plays an important role in prokaryotic evolution and adaptation (ochman et al., 2000; doolittle et al., 2003; kurland et al., 2003).among the various mobile genetic elements that enable this gene exchange, selftransmissible broadhostrange (bhr) plasmids, especially those of the incompatibility.
Rp4 extraction was performed in k12 using a bacterial dna kit according to the manufacturers instructions (omega, usa). plasmid rp4 was added to microcosm for final concentrations of 5 μgml (this concentration is the equivalent of the donor k12 harboring plasmid rp4 at a concentration indicative of conjugative transfer.
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Pro reca hsdr, thi rp42tc::mukm::tn7 integrated into the chromosome plasmids pgs9 cm r, km r, (tn5) p15a replicon ntra tn5 donor psup102 tc r, cm r, vectormobilizable pacyc184 derivative pbluescript sk cloning and sequencing vector, ap r, phagemid, m13 derivative, f1 origin of replication stratagene, la jolla, ca, usa pej1.
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Rp4 gravity concentration tables weigh 60 lbs. and require about 8 to 14 gallons of water per minute. table comes equipped with a 12 hp motor and uses 8.6 amps. 13 hp motor is optional at 6.8 amps. utech shaker table rp4 with. magnetic separator. rp4 operating information.
Rp4 extraction was performed in k12 using a bacterial dna kit according to the manufacturers instructions (omega, usa). plasmid rp4 was added to microcosm for final concentrations of 5 gml (this concentration is the equivalent of the donor k12 harboring plasmid rp4 at a concentration indicative of conjugative transfer), added to.
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Rtpcr was done with 0.5 μg rna in a final volume of 20 μl using the titan onetube rtpcr system according to the manufacturer's instructions (roche laboratories). the annealing temperature used for rtpcr was between 50 c and 58 c, and the cycling conditions were as follows: 94 c for 10 s, 50 to 58 c for 30 s, and 68 c for 30 s.
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